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recJ [2020-04-28 18:35:30]
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recJ [2020-04-28 18:35:30]

single-strand DNA-specific exonuclease, acts together with [protein|E6EA8098E37A4A2F7370920AC1A500EE4319AAA2|RecQ] in replication fork maintenance
locus
BSU_27620
pI
5.18
mw
87.83 kDa
protein length
786 aa Sequence Blast
gene length
2361 bp Sequence Blast
function
replication fork maintenance
product
single-strand DNA-specific exonuclease
essential
no
synonyms
yrvE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.6|DNA repair/ recombination/ based on similarity]
  • [category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins]

    • Gene

    Coordinates
    2,823,419 → 2,825,779

    Phenotypes of a mutant

  • ''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]

    • The protein

    Protein family

  • RecJ family (with [protein|CDFA18E1C0445302ED4D8F93F88822EC6BD646BB|YorK], according to UniProt)

    • Paralogous protein(s)

  • [protein|CDFA18E1C0445302ED4D8F93F88822EC6BD646BB|YorK]

    • [SW|Domains]

  • [SW|DHH-DHHA1 domain]

    • [SW|Cofactors]

  • Mn2+ [pubmed|27058167]

    • Structure

  • [PDB|5F54] (from Deinococcus radiodurans, the [SW|DHH-DHHA1 domain], 32% identity) [pubmed|27058167]

    • [SW|Localization]

  • bound to replication forks, but also assembles at many sites on the nucleoid upon DNA damage induction [pubmed|30401797]

    • Expression and Regulation

    Operons

    genes
    [gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]-[gene|38DF74800FC5D54183C58B0EB82BD9A3EB93EE33|apt]
    description
    [Pubmed|15241682]

    regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

    • regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
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    additional information

  • [protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

    • Biological materials

    Mutant

  • MGNA-B516 (yrvE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1515 NBRP B. subtilis, Japan]
  • GP895 (''[gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]''::''kan''), available in [SW|Jörg Stülke]'s lab
  • BKE27620 (''[gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s labs) [pubmed|28189581]
  • BKE27620 (Δ[gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATTCACCCCTCAACC, downstream forward: _UP4_AGTACGAGGAGGACATAAAA
  • BKK27620 (Δ[gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATTCACCCCTCAACC, downstream forward: _UP4_AGTACGAGGAGGACATAAAA

    • References

    Reviews

  • 22933559,32286623

    • Original publications

  • 15241682,11948165,10498723,21170359,21205011,26001966,27058167,30401797